1. Download cygwin from http://www.cygwin.com/ or http://www.cygwin.com/setup.exe 2. Run setup.exe, choose to install from Internet. The setup will ask you to choose packages to be installed after you choose which site to download. You may need to choose where you want to install cygwin, here we suppose you install it in c:\temp, we call it . In addition to choose the default packages, be sure to choose all sub-categories associated with libtiff under the two categories, Graphics and Libs. If there are other sub-categories about tiff, to choose them will be fine. After the installation of cygwin, it will create a green-with-black icon for cygwin window. The system may need to be restarted. Double click the cygwin window icon, it will open a unix-emulating window. Inside the cygwin window, some popular unix commands can be used: ls df pwd cp Cygwin environment may create a virtual file system, called "/". The windows partition, C: is mounted under /cygdrive/c, and d: is /cygdrive/d. You can use "df" command to check what file systems (or partition) is there. Now, you can correspond d:\shuku\abc.txt to "/cygdrive/d/shuku/abc.txt". Unix's file names are case-sensitive. In the cygwin window, you can use the command "man" to check the usage of a command, like: man cp man ls The start directory of a cygwin window will be "/home/", where user name is your Windows user name. You can check this by type pwd when you just start the cygwin window. 3. Copy the four comands(batch_rfrt, rootflowrt, tensor_robust, mosaic) into "/usr/local/bin" in the cygwin environment. "/usr/local/bin" is chosen here because cygwin's default command parth includes it, so that the user can access the commands without specifying the parth. It is a little confusing to correspond "/usr/local/bin" in Windows explorer, it is actually in \cygwin\usr\local\bin under windows system, if is c:\temp, then it is c:\\temp\cygwin\usr\local\bin. The confusion comes from the situation that the sub-folder in the cygwin environment, /cygdrive/c, is containing the parent folder /, bcause the root / in cygwin environment is actually under c:\temp\cygwin in Windows environment. You can just forget the confusion here. In cygwin window you can use command in cd c: to go to c:. 4. To run the plant root image sequence, you can create a working directory, say c:\plantroot\root1, corresponding to /cygdrive/c/plantroot/root1. Then you create sub-folders under it, curve, pgm, intermediate, tiff. You put the root image seuqences into tiff sub-folder, with the file names like "Stack00##Frame 00##". In the cygwin window, type the following commands: cd c: cd plantroot/root1 batch_rfrt . < input.txt Then RootflowRT will be launched.